Human papillomavirus genotypes in human immunodeficiency virus-positive patients with anal pathology in Madrid, Spain.

Benjamín García-Espinosa, Ernesto Moro-Rodríguez and Emilio Álvarez-Fernández

Background: We studied anal specimens to determine the distribution of human papillomavirus (HPV) genotypes and co-infection occurrence. This information will contribute to the knowledge of HPV genotype distributions and provide an estimate of the prevalence of different oncogenic HPV genotypes found in patients in Madrid (Spain). Methods: We studied a total of 82 anal biopsies from the Hospital General Universitario Gregorio Marañón of Madrid. These included 4 specimens with benign lesions, 52 specimens with low-grade anal squamous intraepithelial lesion, 24 specimens with high-grade anal squamous intraepithelial lesions and 2 specimens with invasive anal carcinoma. HPV genotyping was performed with PCR amplification and reverse dot blot hybridization. Results: We detected 33 different HPV genotypes, including 16 HPVs associated with a high risk of carcinogenesis, 3 HPVs associated with a highly likely risk of carcinogenesis and 14 HPVs associated with a low-risk of carcinogenesis. In two specimens, an uncharacterized HPV genotype was detected. The most frequent HPV genotypes found were HPV-16 (10.3%; 95% CI: 6.6%-15.1%), HPV-52 (8.5%; 95% CI: 5.2%-13%) and HPV-43/44 (7.6%; 95% CI: 4.5%-11.9%). HPV-18 was only detected in 0.9% (95% CI: 0.1%-3.2%) of the total viruses detected in all lesions. HPV co-infections were found in 83.9% of all types of lesions. The majority of cases (90.2%) were concomitantly infected with the human immunodeficiency virus (HIV). Conclusion: The prevalence of high-risk carcinogenic genotypes in anal pathological samples was remarkable. Therefore, further studies that include a greater number of samples, particularly invasive carcinoma cases are needed to evaluate the potential influence of these HPV genotypes in the appearance of anal carcinomas. Also, the influence of other accompanying infections should be evaluated clarify the appearance of this type of carcinoma.

Diagnostic Pathology 2013, 8:204 doi:10.1186/1746-1596-8-204

Published: 10 December 2013

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Estafas de tratamiento contra el cáncer | Información para consumidores

http://www.consumidor.ftc.gov/articulos/s0104-estafas-de-tratamiento-con...

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Human papillomavirus-associated oral intraepithelial neoplasia.

Sook-Bin Woo, Emma C Cashman and Mark A Lerman

Abstact: This study evaluated an unusual subset of oral epithelial dysplasia for the presence of transcriptionally active high-risk HPV subtypes and to further characterize the histological criteria for this condition. There were 20 cases diagnosed as epithelial dysplasia with marked apoptosis of the anterior oral cavity. Clinical and follow-up data were collected and histopathological features were documented. Immunoperoxidase studies were performed for p16 and in situ hybridization studies were performed for low- and high-risk HPV sub-types. Gender- and site-matched controls of conventional moderate-to-severe oral epithelial dysplasia were similarly evaluated using immunoperoxidase studies for p16 and in situ hybridization; the number of apoptotic cells for study and control cases was counted at two different tissue sites. There were 17 men and 3 women with a median age of 56 years. Seventeen lesions were described as white and five were described as rough or papillary. Thirteen were located on the lateral or ventral tongue, some extending onto the floor of the mouth. Epithelial hyperplasia with marked karyorrhexis and apoptosis were present in all the cases, along with features of conventional oral epithelial dysplasia. A statistically significant number of apoptotic cells were identified in the study cases when compared with controls (P>0.0001). Twenty cases were positive for high-risk HPV by in situ hybridization and all 19 nineteen cases evaluated for p16 demonstrated overexpression. Two patients were diagnosed with squamous cell carcinomas and one patient developed recurrent disease. We report a subset of oral epithelial dysplasia that occurs mostly in adult men on the ventral or lateral tongue and is positive for high-risk HPV and for p16. We propose use of the term ‘HPV-associated Oral Intraepithelial Neoplasia’ to characterize these lesions of the oral cavity for consistency in nomenclature with HPV-associated lesions of the lower anogenital tract. One case recurred and one developed invasive cancer.

Modern Pathology 26, 1288-1297 (October 2013) doi:10.1038/modpathol.2013.70

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11. november 2013

EUROPALS pathology test

To all members of the ESP

From November 25th we offer the 2013 EUROPALS pathology test intended for trainees (but pathologists are free to participate as many as are interested). The test consists of 100 questions, partly straight multiple choice questions, partly still image based and partly virtual slide based, and extended matching questions.

Fuente: Cancer.gov - NCI HPV can damage genes and chromosomes directly - National Cancer Institute

Genome-wide analysis of HPV integration in human cancers reveals recurrent, focal genomic instability

Screening for HPV May Be Better Than Pap Test, Study Suggests: MedlinePlus

Efficacy of HPV-based screening for prevention of invasive cervical cancer: follow-up of four European randomised controlled trials

Guglielmo Ronco, Joakim Dillner, K Miriam Elfström, Sara Tunesi, Peter J F Snijders, Marc Arbyn, Henry Kitchener, Nereo Segnan, Clare Gilham, Paolo Giorgi-Rossi, Johannes Berkhof, Julian Peto, Chris J L M Meijer. The International HPV screening working group†

NIH VideoCasting and Podcasting

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Serie de Antena3 -- Física o Química - Marina tiene VIH Un reflejo en los medios de comunicación de cómo parece ver la sociedad española esta enfermedad.

NEJM Dialogues Second centenary -- HIV Panel

NEJM - Risk of Cancer in a CT-Discovered Nodule

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EDUMEDIC - 10 Free Google Chrome Extensions Teachers Should Try

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Updated Debian 7: 7.2 released

12 de octubre 2013

Human Papillomavirus Prevalence in Invasive Anal Cancers in the United States Before Vaccine Introduction

Steinau, Martin; Unger, Elizabeth R.; Hernandez, Brenda Y. ; Goodman, Marc T.; Copeland, Glenn; Hopenhayn, Claudia; Cozen, Wendy; Saber, Maria S.; Huang, Youjie; Peters, Edward S.; Lynch, Charles F.; Wilkinson, Edward J.; Rajeevan, Mangalathu S.; Lyu, Christopher; Saraiya, Mona

Eusalud se adhiere a la Declaración de la sociedad civil con ocasión del XX Aniversario del año internacional de la familia

Fuente: The Family Watch. Instituto Internacional de Estudios sobre la Familia

Para Manifiesta su voluntad de adherirse a la Declaración. Siga el vínculo y rellene el formulario que aparece al pié de página

La Declaración de la sociedad civil propuesta por buena parte del conjunto de organizaciones internacionales relacionadas con la familia incluye los contenidos básicos de las resoluciones de Naciones Unidas sobre el Año Internacional de la Familia, así como las principales recomendaciones de los distintos Encuentros de Expertos, en especial el Europeo que hemos organizado en Bruselas. Buscamos para ella la adhesión de cuantos políticos, académicos y representantes de ONGs podamos reunir, de manera que pueda presentarse junto a las demás organizaciones internacionales en la Comisión de Desarrollo Social que tendrá lugar en febrero de 2014. Esperamos también que su contenido pueda formar parte de una resolución de la Asamblea General de NN UU con ocasión de este Aniversario.

Cursos on-line en MOOC: 150 cursos universitarios, online y gratuitos que inician en Octubre

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Pathpedia: Database of immunohistochemical markers

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